|Tower 3||Sacred Land|
- The Underwater Ruin, an Area which wasn't present in MHP3rd, returns in this game.
- Monster Hunter 3 Ultimate uses the Monster Hunter Tri version of the Flooded Forest rather than MHP3rd's version.
- Tower 3 from Monster Hunter Freedom Unite reappears in MH3U.
- The Misty Peaks Area is the only new Area from Monster Hunter Portable 3rd that returned.
- A new Town, Port Tanzia, replaces Loc Lac City.
- Tainted Sea is a new Area where hunters battle against Dire Miralis.